We want to sterilize a medium prepared with epidermal growth factor (among other components) but we are not sure if it can be filtered throw 0.22 µm.
Autoclave is not suitable since medium contains glucose.
hi
ok. you can used low protein binding syringe filter 0.2 um ( such as milipore brand) .
good luck
hi
ok. you can used low protein binding syringe filter 0.2 um ( such as milipore brand) .
good luck
I second Reza's opinion. A 0.22um polyethersulfone filter should do it. Personally, I like CellTreat. Cheap and does the job. While PES filters aren't very resistant to...well anything you wouldn't put on cells, they are perfect for filter sterilizing media since PES is extremely low binding to proteins. SFCA will also give similar results to PES, though in my experience they are a bit stickier to proteins while giving you a bit more resistance to other chemicals.
Of course, mycoplasma and viral particles will still get through.
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