Elisabet Pérez-Albaladejo

3 Questions 3 Answers 0 Followers

Questions related from Elisabet Pérez-Albaladejo

Can a culture medium containing Epidermal Growth Factor (EGF) be sterilized by using a 0.22 µm filter?

We want to sterilize a medium prepared with epidermal growth factor (among other components) but we are not sure if it can be filtered throw 0.22 µm. Autoclave is not suitable since medium...

25 October 2016 4,200 3 View

Is Trizol reagent suitable to be used directly in the 12-well culture plates?

I need to extract mRNA from my cells but I am not sure if Trizol, a corrosive product, is compatible with the culture 12-well plates. May I should trypsinize and transfer the cells to a eppendorf...

31 August 2016 1,962 4 View

How I can test the ROS production in animal cells?

I usually use the H2DCF-DA assay but I would like to try another method because I have strange results and I would like to confirm with another test.

17 December 2015 6,940 2 View