Colin Germer

6 Questions 26 Answers 0 Followers

Questions related from Colin Germer

What Is An Optimal Extracellular Cryoprotectant/Filler For HPF?

Hi, Perhaps I've been searching wrong, but I've been unable to find a satisfactory answer for a good extracellular cryoprotectant in high pressure freezing. I know there are many options but each...

08 January 2018 3,634 5 View

Should you mix osmium and cacodylate?

Hi all, This is a question that's recently popped up. I've been working to optimize my TEMs to get good ultrastructure and checked out more than a few books from my institution's libraries to see...

26 May 2017 8,598 16 View

Can Cacodylate Rinses Before Fixation Affect Cell Ultrastructure?

Hi, I'm in the midst of troubleshooting TEM. My cells are polarized epithelia grown on Transwells. Polyester membranes are used since polycarbonate is degraded by propylene oxide, ruining the...

03 March 2017 8,139 3 View

How can I prevent aggregation of exosomes?

Hi, I'm performing DLS on exosomes. For the most part, I'm getting very good, very tight reads consistent with the known size of exosomes! However, I do get a spike in the 1um range. I know there...

25 October 2015 9,704 17 View

How to determine density gradient?

Hi, I'm using density gradients to isolate exosomes! I've read that they appear in the 1.11g/mL fraction. Following in the steps of a few authors, I created a self-generating density gradient by...

25 September 2015 1,521 6 View

Do Exosomes Need to be Lysed before SDS-PAGE?

I'm using the Total Exosome Isolation Kit (from cell culture media) by Invitrogen and comparing it to ultracentrifugation. However, I can't seem to get any bands in the TEI kit during Western. I'm...

18 September 2015 7,329 17 View