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Questions related from Colin Germer
Hi, Perhaps I've been searching wrong, but I've been unable to find a satisfactory answer for a good extracellular cryoprotectant in high pressure freezing. I know there are many options but each...
08 January 2018 3,641 5 View
I've been looking around and I can't seem to find a satisfactory answer on this subject. I understand that an SDCM already performs deconvolution by mere virtue of existing (pinholes ignore...
18 August 2017 7,989 3 View
Hi all, This is a question that's recently popped up. I've been working to optimize my TEMs to get good ultrastructure and checked out more than a few books from my institution's libraries to see...
26 May 2017 8,603 16 View
Hi, I'm in the midst of troubleshooting TEM. My cells are polarized epithelia grown on Transwells. Polyester membranes are used since polycarbonate is degraded by propylene oxide, ruining the...
03 March 2017 8,144 3 View
Hi, I'm performing DLS on exosomes. For the most part, I'm getting very good, very tight reads consistent with the known size of exosomes! However, I do get a spike in the 1um range. I know there...
25 October 2015 9,708 17 View
Hi, I'm using density gradients to isolate exosomes! I've read that they appear in the 1.11g/mL fraction. Following in the steps of a few authors, I created a self-generating density gradient by...
25 September 2015 1,526 6 View
I'm using the Total Exosome Isolation Kit (from cell culture media) by Invitrogen and comparing it to ultracentrifugation. However, I can't seem to get any bands in the TEI kit during Western. I'm...
18 September 2015 7,333 17 View
