CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How do you integrate undergads into research?

I'm passionate about mentoring undergrads into the research process--not just collecting data, lit review, but also analysis. How do others go about working with undergrads in research? How do you...

30 November 2020 3,138 6 View

In Ansys HFSS, how do I replace all instances of a Local Variable within a Design with another variable?

I have created a fairly complex antenna that has a parameterized wire radius (wire_r), however I would like to change the name of the variable to better reflect its purpose (e.g. wire_r_cu). Since...

04 November 2020 7,168 1 View

In Ansys HFSS, is there a method of creating an oscillating (AC) magnetic bias, without resonant peaks, within a non-magnetic substrate, e.g. Air?

A loop sniffer probe antenna for HF/UHF is being developed and we'd like to apply a known, constant across frequency AC B-Field source to the region within the substrate where the probe exists (in...

01 November 2020 5,717 3 View

Is it advisable to dichotomize an independent variable measured using a Likert scale?

I am working on a research project to identify the relationship between sexual esteem and sadistic behavior. Both of my independent and dependent variable have been measured using standard...

16 April 2020 7,062 4 View

Survey Data Anlysis/Testing Support - any help?

Hi - hoping someone might be able to help me please? I've had to totally change my MSc project as a result of COVID and as I can no longer do face to face testing, I have created a survey and...

14 April 2020 6,292 9 View

How is it possible to detect that my UPL probe is expired or does not work?

Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...

20 January 2020 2,431 1 View

How to preserve endogenous GFP/RFP during IHC?

I have a dual reporter mouse that expresses RFP and GFP under two promoters. I would like to also do IHC on this tissue but the antigen retrieval step (heat) denatures the GFP. Moreover, if I skip...

11 December 2019 403 3 View

Good example of building communication theory using research data?

A colleague and I are working on developing a communication theory. This of course synthesizes prior literature, but we also have survey data we gathered to help us "make the case" for how the...

06 October 2019 1,048 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View