When selecting a stably transfected clone, does the clone appear to lose differentiation capability? I have had experience when growing C2C12 cells that they start to differentiate at a high confluency even without the differentiation medium. This supposedly depletes the myoblast population. So I am just wondering how people think about balancing the need to expand the clone with preventing loss of differentiation capacity.
Well it depends on what you're transfecting into C2C12; different plasmids and siRNAs can induce differentiation. If you're doing a proper stable transfection (e.g. with https://altogen.com/product/c2c12-transfection-reagent-mouse-myoblast-cells/) then you'll end up with the cells expressing some protein at substantial amounts, which can change the way the cells behave, and consequently differentiate. With regards to larger colony sizes, just make sure to passage the cells frequently and you should be fine.
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