2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View

TRAP staining (rat femur) faids away ovenight?

Hi, does anyone know why TRAP staining faids in FFPE rat femur sections? My protocol: After deparaffination and rehydration i put the slides in Acetat Buffer for 20min, then they go to the...

14 December 2020 3,112 4 View

Can anyone help me regarding the doubling time of JAWS II cells?

Anyone with experience in growing JAWS II cells could give me an idea of its doubling time? I intend to use these cells for transfection protocols. Any tips to help with growing and transfecting...

01 December 2020 855 3 View

Which software, other than NVivo, do you use to code and perform thematic analysis of your interviews?

We undergone so much problems with NVivo (activation, compatibility PC vs MAC) and it is so much expensive.

07 July 2020 8,222 10 View

Secondary structure causing late amplification in NTC of TaqMan qPCR?

Hello, Excluding contamination and probe degradation, can anyone suggest a cause of late amplification in the NTC of TaqMan qPCR ? I considered the possibility of primer dimers, but since they...

21 March 2020 8,904 3 View

What can I do to improve my 260/230 values?

I am experiencing low 260/230 values after using Qiagen's DNeasy PowerSoil Pro Kit. I have been getting values anywhere from around 0.11 to 1.10. After speaking with a Qiagen rep, she recommended...

03 March 2020 4,203 7 View

Can 4% PFA in methanol free PBS fix AND permeabilize cells ?

Dears, I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet). No I would like to stain lipid droplets in the cell with oil-red solution. I thought I will NEED...

06 February 2020 2,010 1 View

Doubt about anti-mouse IgG-CFL 488 sc-362259 specificity?

I am using the rabbit anti-mouse IgG-CFL 488 sc-362259 from Santa Cruz and because some problems we performed a test using the secondary antibody without the primary and we realized this antibody...

15 January 2020 2,947 1 View

Is there any maximum number for studies that can be included in meta-analysis?

I'm doing a meta-analysis under a broad topic and about 50 studied reached my inclusion criteria. Is that number acceptable and if not how many studies should I have? Indeed, the topic of this...

12 September 2019 1,644 4 View

How do I find km and vmax with and allosteric enzyme?

While doing research on an enzyme one of my graphs was sigmoidal and I need to find km and vmax however I can't find a good article to explain how to do this since the graph doesn't follow the...

19 June 2019 3,793 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

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02 March 2021 3,060 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View