How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

Where can I find SAR standard Image data sample for image registration?

I want to register some SAR Images so I need standard data which is commonly used by research community for comparison sake. My question is that does such standard data exist for comparing tasks...

31 October 2020 1,913 5 View

How can I induce colon carcinoma cancer in mice?

I want to try to induce colon cancer in mice, and then evaluate potency of anticancer drug in these mice. It means I will have to evaluate size of tumor following injection of antitumor drug in...

28 August 2020 9,040 3 View

How to prepare carbon paste as electrode for Pervoskite solar cell?

I am working on perovskite solar cell and want to use carbon as an electrode but I don't know how to prepare carbon paste. Please suggest me some easy methods or related publications.Thanks

24 April 2017 893 3 View

How to transform data for multilabel classification, when using NN?

12 September 2016 2,882 5 View

How can I obtain a reaction force of the contact?

10 November 2014 9,519 9 View

Tips for Coating ELISA plate with antibodies?

I am developing a sandwich ELISA, for that I need to coat antibodies on plate. I need your suggestions about coating buffer, dilution factor, and blocking buffer. Someone told me you need a...

26 January 2014 9,819 6 View

Buckling of a structure ?

Neglecting geometric and material non-linearities, are post-buckling deformations necessarily within the nonlinear stress-strain regime? I am trying to see if buckling forces are a good gauge to...

01 January 1970 4,155 3 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do I apply continuity boundary condition at the edge using form assembly in comsol?

The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...

05 February 2021 3,459 2 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View