Can a compound has two emission peaks with one excitation wavelength?

Greetings, I do some research regarding molecule structure embed with triazole moiety, there is two emission peaks observed in flurorescence. How could these observation happened from triazole ?...

09 October 2018 3,563 7 View

How pH can be a good indicator to self aggregation or phase transition?

Good morning to those who reading this question. I would like to have any references about aggregation of surfactant by triggering the conditions of system using pH. Besides, how pH can help to...

08 September 2018 7,399 3 View

How to calculate mixed surfactants using Rubingh theory?

Greetings, I have a problem how to use the Rubingh theory equation in my reserach. I have the equation and I could not totally understand about how to insert the value in the equation. May you...

06 July 2018 4,642 0 View

How to choose whether single or double beam mode in UV-VIS?

Hi, I am using Cary 60 UV Vis, and at the setup in the instrument, it has option to use single or double beam mode. May I know which one is better? And how to operate for each beam mode? For...

01 February 2018 3,553 3 View

In mixture of anionic and nonionic surfactant, what effect of pH can happen to the mixture ?

If I have a mixture of anionic and non-ionic surfactants that will mix together, what are pros and cons if I change the pH of concentration at high pH (pH 12) compared to slightly low pH (maybe...

31 December 2017 7,703 3 View

Choosing a buffer solution?

02 March 2017 2,225 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View