Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

in concrete application Which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

My dear colleagues, in concrete application which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

19 April 2019 4,501 8 View

What is the best way to of the waste plastic fiber to enhance some of the mechanical properties of concrete?

Dear Colleagues What is the best way to enhancing the shape and the outer surface of the waste plastic fiber to enhance some of the mechanical properties of concrete?

21 September 2018 7,603 1 View

Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

Dear Colleagues Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

31 May 2018 5,727 6 View

Polymer diposition on nanoparticles?

I wanted to diposite a polymer on nanoparticles,but the TEM results shows that that the polymer diposite on an aggregated nanopartilces together. is that a problem of the nanoparticles dispertion...

04 April 2017 204 5 View

How I can calculate vertex coordinates from vertex normal in triangular mesh ?

I have triangle mesh and calculate normal of triangles then calculate vertex normal and do some calculations on it and want to calculate vertex coordinates from this vertex normal after do...

01 November 2016 992 3 View

How to measure catalase activity in a plant extract?

can you describe please the contents of a simple reaction to measure catalase activity in a plant extract and is it possible to be a water extract or should it be in phosphate buffer? thanks a lot

05 April 2016 9,175 3 View

Standards in ELISA??

28 February 2016 9,668 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View