Why unexpected peak before the standard pesticide peak in HPLC?

I worked with procymidone and its metabolites for residue analysis in fruits and vegetables. But, when I injet the stardard vials of procymidone for the calibration curve there is a giant...

08 September 2019 8,845 8 View

Concentration of hexane:ethyl acetate in rotary evaporator?

I use hexane:ethyl acetate (6:4) as solvent for extraction of pesticide from the spiked amount. The pesticide Hexaconazole in acetonitrile (5 ppm) is my spiked pesticide. After final...

02 March 2019 4,439 3 View

Is QuEChERS clean up technique suits for both LC-UVD and LC-MS/MS?

In the recent research articles, researchers uses QuEChERS techniques of extraction and clean up for the plant samples and other food samples with a customized modification. But, they using...

31 December 2018 4,514 8 View

What is the PLS in pesticide use in crops?

In a recent conference I attended, the main theme is PLS of pesticides for minor crops and there is counter talk between PLS and Zero Tolerance. But, due to language barrier I have some...

09 October 2018 7,751 0 View

How to fit the Double Exponential (DE) model for non linear fit?

Double exponential (DE) is a popular non linear model for calculating kinetic degradation of organic molecules/pollutants. In DE model, the two distinctive phase of degradation (fast and slow) is...

08 September 2018 9,882 7 View

What is the Unit of enzyme Laccase?

Copper containing enzyme Laccase unit is based on spectrometric data. How to measure 1 unit laccase for the experimental use. Any reference?

06 July 2018 3,229 8 View

Requesting a Chapter of SSSA Book Series?

Dear Fellow Researcher, have you access in this article https://dl.sciencesocieties.org/publications/books/pdfs/sssabookseries/pesticidesinthe/169

04 May 2018 4,497 0 View

Is QuEChERS extraction in LC-MS/MS shows best recovery analysis?

Looking for any article describing the detailed protocol and published in a smart impact journal having IF 4 or above.

04 May 2018 2,882 5 View

Impact factor vs Citation (Open access Indexing), which is better for a new researcher?

Google scholar citation/H index/High IF? Amonst these which one is most prferable for a beginner in the research journey.

04 May 2018 6,199 1 View

DT50 and Half life of Pesticides are same? How to measure these values? Is first order kinetics suits all time with field data?

Searching for any suitable and reliable protocol for the calculation of DT50 of some Pesticides.

02 March 2018 5,285 10 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View