I use hexane:ethyl acetate (6:4) as solvent for extraction of pesticide from the spiked amount. The pesticide Hexaconazole in acetonitrile (5 ppm) is my spiked pesticide.
After final concentration in rotary evaporator followed by florisil open column; the recovery was very low (less than 60%) with a higher amount of peak area of solvent in the LC-UVD peaks. Now, I am looking for tips and technique for proper concentration of ethyl acetate by rotary evaporator.
Expecting experts kind contribution in this regard.
-Aniruddha Sarker
Can you describe all stages of your procedure in details?
regards,
GB
Solubility of Hexaconazole In water 0.017 g/lit. at 20 C. in Methanol 246, Acetone 164, ethyl acetate 120, toluene 59 , hexane 0.8 (all in g/l at 20°C ) so the solubility in hexane:ethyl acetate is low . try to extract using other solvents like methanol .
Grzegorz Boczkaj Thank you for your kind interest. The temp of rotary evaporator is fixed at 40 °C with vacuum pressure of 120 psi. And H:EtOAc 6:4 solvent is the extracting solvent in florisil open column. The extracted pesticide is 5 ppm spiked Hexaconazole in acetonitrile.
But in LC-UVD peak, there is a visible unexpected solvent peak. How to reduce this unexpected peak of solvent?
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