Databases for protein?

Hello All. I have been working on a set of proteins related to cancer initiation and progression. I wish to know the databases wish may provide me the information for the status of any protein...

01 February 2016 5,776 6 View

ShRNA mediated knockdown showing effects in the protein level but not in the RNA level, what might be the reason?

04 May 2015 5,554 7 View

What is the approximate time for mammalian cells that have been synchronized for a particular phase to become asynchronous again?

Does it depend on the phase in which it has been blocked, i.e. G0/G1 will take one time while G2/M will have another? Does it depend on the cell's type (dividing cells) or the cells lines one is...

04 May 2013 9,463 3 View

Flag tagged clone not expressing.

I had cloned my gene of interest (GOI) into pCMV10-3X-Flag vector for expressing into mammalian cells. Even though the gene has been cloned and sequence confirmed, I am having a problem with...

04 May 2013 4,483 10 View

Protein binding too tightly to the beads.

I have been trying to elute protein of my interest tagged to GST using a protocol containing 10mM reduced glutathione buffered at pH 8.0. However, contrary to the proposed protocol for this...

04 May 2013 272 10 View

Finding new interacting partners for my protein of interest (POI).

I want to find new interacting partners for my protein of interest(POI), but I do not want to follow the more conventional and time taking methods like the yeast two hybrid system which also may...

31 December 2012 1,140 9 View

What causes a protein to get degraded or loose its activity if stored for a long period at 4 degrees?

I had purified a kinase of my interest to near homogeneity and had stored it at 4 degrees for approximately a month to carry out assays with it. However, I found the kinase activity to have been...

31 December 2012 6,140 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View