Arnab Bose

8 Questions 18 Answers 0 Followers

Questions related from Arnab Bose

Databases for protein?

Hello All. I have been working on a set of proteins related to cancer initiation and progression. I wish to know the databases wish may provide me the information for the status of any protein...

02 February 2016 5,816 6 View

ShRNA mediated knockdown showing effects in the protein level but not in the RNA level, what might be the reason?

Hello everyone, I have been trying to standardize the knockdown of my gene of interest with the shRNA bought from DHARMACON, which targets the coding sequences. Though I get reproducible results...

05 May 2015 5,596 7 View

What is the approximate time for mammalian cells that have been synchronized for a particular phase to become asynchronous again?

Does it depend on the phase in which it has been blocked, i.e. G0/G1 will take one time while G2/M will have another? Does it depend on the cell's type (dividing cells) or the cells lines one is...

05 May 2013 9,505 3 View

Protein binding too tightly to the beads.

I have been trying to elute protein of my interest tagged to GST using a protocol containing 10mM reduced glutathione buffered at pH 8.0. However, contrary to the proposed protocol for this...

05 May 2013 313 10 View

Blocking after transient transfection?

I want to block HEK293T cells in the G2/M phase after transfecting them with the construct of my interest via a double thymidine/nocodazole block. Does anyone have a protocol or suggestion...

05 May 2013 4,701 1 View

Flag tagged clone not expressing.

I had cloned my gene of interest (GOI) into pCMV10-3X-Flag vector for expressing into mammalian cells. Even though the gene has been cloned and sequence confirmed, I am having a problem with...

05 May 2013 4,522 10 View

Finding new interacting partners for my protein of interest (POI).

I want to find new interacting partners for my protein of interest(POI), but I do not want to follow the more conventional and time taking methods like the yeast two hybrid system which also may...

01 January 2013 1,175 9 View

What causes a protein to get degraded or loose its activity if stored for a long period at 4 degrees?

I had purified a kinase of my interest to near homogeneity and had stored it at 4 degrees for approximately a month to carry out assays with it. However, I found the kinase activity to have been...

01 January 2013 6,182 11 View