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Questions related from Arnab Bose
Hello All. I have been working on a set of proteins related to cancer initiation and progression. I wish to know the databases wish may provide me the information for the status of any protein...
02 February 2016 5,817 6 View
Hello everyone, I have been trying to standardize the knockdown of my gene of interest with the shRNA bought from DHARMACON, which targets the coding sequences. Though I get reproducible results...
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Does it depend on the phase in which it has been blocked, i.e. G0/G1 will take one time while G2/M will have another? Does it depend on the cell's type (dividing cells) or the cells lines one is...
05 May 2013 9,506 3 View
I have been trying to elute protein of my interest tagged to GST using a protocol containing 10mM reduced glutathione buffered at pH 8.0. However, contrary to the proposed protocol for this...
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I want to block HEK293T cells in the G2/M phase after transfecting them with the construct of my interest via a double thymidine/nocodazole block. Does anyone have a protocol or suggestion...
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I had cloned my gene of interest (GOI) into pCMV10-3X-Flag vector for expressing into mammalian cells. Even though the gene has been cloned and sequence confirmed, I am having a problem with...
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I want to find new interacting partners for my protein of interest(POI), but I do not want to follow the more conventional and time taking methods like the yeast two hybrid system which also may...
01 January 2013 1,177 9 View
I had purified a kinase of my interest to near homogeneity and had stored it at 4 degrees for approximately a month to carry out assays with it. However, I found the kinase activity to have been...
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