Does it depend on the phase in which it has been blocked, i.e. G0/G1 will take one time while G2/M will have another? Does it depend on the cell's type (dividing cells) or the cells lines one is looking at?
varies by cell line. Do the experiment, taking samples each day. Analyze for DNA content, cell cycle modeling by flow cytometry.
Interesting question.
Firstly some data from a setting without chemical synchronisation: I observed individual HeLa cells over prolonged times. On average, sibling cells become asynchronous in their next division by about 80 min (>160 cell pairs analysed) - Rehm M et al, Cell Death Differ 2009.
For phase-based synchronisation you'll need to read this paper: Cooper S. Rethinking synchronization of mammalian cells for cell cycle analysis. Cell Mol
Life Sci 2003; 60: 1099–1106.
This really well discusses the implications of different phase-arrests with respect to the synchrony that can be achieved.
Hope this helps!
Generally, one cell cycle is a reasonable assumption. However, as Jonathan mentioned this can be highly cell specific. If the cell line has a mutation in cell cycle checkpoint proteins or so they will leak. Here attached is a provocative view of cell synchronization.
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