01 January 1970 3 8K Report

Hi!

I am trying to select the lowest amount of blasticidin needed for selection in murine primary neurons. I have tried concentrations from 1-8 ug/ml and had no difference in the fitness between the cells. The cells did appear dead, the axons were disintegrating but the cells didnt actually detach from the wells.

I am not sure what to make out of this, could I use blasticidin for selection and passage the cells & count on that the dead cells wont attach but that the live ones will?

Any input would be appreciated.

Best,

Linn

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