How do I blot an 8kDa protein?

Hi, I am trying to blot a protein of 8kDa with western blotting. So far I have tried to use 0.45um & 0.2um nitrocellulose membranes for transfer as well as 0.2um PVDF. I have tried to use...

09 October 2019 7,470 5 View

Enrichment of spermatogonial stem cells from C57BL/6 mice?

Dear reader, I am searching for a protocol on isolation and enrichment of spermatogonial stem cells (SSCs) that is suitable for C57BL/6 mice without a mixed background. As far as I have been...

08 September 2018 7,226 3 View

How to transfect & FACS sort primary murine neurons?

I am currently isolating and cultivating primary neurons from E18.5 C57BL/6 mice. I am planning to transfect them with both CRISPRs and a selection vector that will express RFP when the Cas9 is...

01 January 1970 2,901 5 View

Blasticidin selection in murine primary neurons

Hi! I am trying to select the lowest amount of blasticidin needed for selection in murine primary neurons. I have tried concentrations from 1-8 ug/ml and had no difference in the fitness between...

01 January 1970 7,693 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View