Hi all,
I am a bit confused with the following situation: I am interested in expression levels within cells upon treatment. I transfected one dish of cells and used a small amount of them for imaging. I'm measuring expression for 30 cells each (control/treatment). I'm not really sure how to deal with this sample in terms of statistics. Is it possible to think of every single cell as a biological or technical replicate? For me, both don't make 100% sense.
I'd be very happy about any suggestions!
Hi Laura! To ensure statistical robustness, I strongly suggest that technical replicates should come from multiple wells within the same experiment, while true biological replicates require independent experiments conducted on different days with separate cell cultures and transfections. it would be desirable performing at least three independent biological replicates. Using pseudo-technical replicates introduces the risk of overestimating statistical significance if the variability between independent experiments is not properly accounted for.
There's a good discussion of these issues (and guidelines for how to present your data clearly) in this paper: Article SuperPlots: Communicating reproducibility and variability in...
Thanks to all of you for your replies, really appreciate it!
I was wondering what you think about stable cell lines? If I want to show an effect of a K.O. cell line and one that stately expresses XY, do I just take samples at 3 different time points? Or do I need to repeat stable transduction 3 times?
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus