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Questions related from Laura Charlier
I am doing lots of Western Blots and never had any major issues with it. However, I have been struggling to get a nice transfer for a protein of 150 kDa. In the attached figure you see lysates of...
05 April 2025 4,401 3 View
Hi all, I am using FuGENE to transiently transfect RPE1 cells for imaging. I usually seed them on coverslips and transfect them when they have reached 60-70% confluence. For 24-well dishes I use...
03 March 2025 3,373 1 View
I am doing a GST-pulldown. For this, I am coupling GST magnetic agarose beads to my GST fusion proteins; after that I incubate this mixture with my cell lysates. I've made a mistake and started to...
01 February 2025 8,188 3 View
Hi everyone, I'm currently using AI to predict binding interfaces in a protein complex. I used models from AlphaFold2, Rosetta2 and ChaiDiscovery. I analyzed the models with Chimera using the...
12 December 2024 709 1 View
Hello, I'm doing site directed mutagenesis. So far, I didn't have any issues. For one construct only, I get insertions that are equal to my primer sequences. Sometimes it is 1, sometimes it's 3...
05 December 2024 7,861 3 View
I sent in my prepped plasmids for DNA sequencing. Unfortunately, the results are very noisy (I attached a screenshot). I double-checked and the samples have the right concentration (100 ng/ul) and...
01 January 1970 6,461 9 View
Hi all, I am a bit confused with the following situation: I am interested in expression levels within cells upon treatment. I transfected one dish of cells and used a small amount of them for...
01 January 1970 2,978 4 View
