Tris-Glycine Transfer Buffer (1X Formulation: 25 mM Tris, 192 mM Glycine, 20% (v/v) methanol, 0.03% sodium dodecyl sulfate (SDS) pH ~8.3) for PVDF without SDS for NC. or 10 mM CAPS, pH 11 and 10% methanol.

exactly Jarosław Króliczewski  sir