Is fluoro-jade staining possible with tissue-cleared brains?

I am hoping to stain whole mouse brain hemispheres with fluoro-jade C as a marker of degeneration. We are presently working to optimize this staining for tissue cleared with the LifeCanvas SHIELD...

10 March 2020 9,709 1 View

Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

09 February 2020 6,330 5 View

Does it help for a prospective graduate student to have a research proposal in hand when applying to grad school?

This coming semester, I will be teaching an undergraduate class on graduate school preparation for science majors. One of the main assignments during the class will be the assembly of a full...

17 December 2019 8,239 11 View

Looking for validated questionnaires to use with both children and parents on reporting Emotional Health/difficulties?

Hi there, I'm currently doing my MSc in Psychology of Education. I'm looking to answer the following research questions for my dissertation? a) Are there differences in self-reporting of...

13 December 2019 8,779 5 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

02 November 2017 9,029 3 View

How do you respond to requests for article reprints for articles which were published in journal to which you assigned copyright?

If the publication journal 'owns' the article and the request is not for teaching/educational purposes from someone who is not a current collaborator, do such requests fall under 'fair use' or...

17 October 2017 5,325 3 View

Has anyone conducted research on child protection, self harm, sucide, etc in SE Asia, more specifically Laos if possible?

Looking for any research around child protection in Laos. GoL sensors everything so I'm looking for information that might not have been censored yet. Thank you!

16 August 2017 614 3 View

Can SPSS be used to carry out a Cox PH model with a time-varying exposure covariate that changes status more than once?

I have looked through some help documents that indicate that the time-varying covariate can be constructed for an individual that has been exposed and then unexposed, or vice-versa. I was...

26 July 2017 7,097 3 View

ANOVA versus Student's t-test_Small samples

Hi, I hesitate between two statistical tests (ANOVA and Student's t-test) to compare the means of three groups (unpaired, n

20 July 2017 6,168 6 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View