Rogan A Grant

4 Questions 6 Answers 0 Followers

Questions related from Rogan A Grant

Best way to sterilize percoll solutions?

I tend to prepare percoll dilutions in bulk (40% in HBSS in this case, but occasionally we use 30% and 70%) for large experiments and filter sterilize the finished product with a 0.22µm filter....

25 March 2021 1,339 0 View

Is fluoro-jade staining possible with tissue-cleared brains?

I am hoping to stain whole mouse brain hemispheres with fluoro-jade C as a marker of degeneration. We are presently working to optimize this staining for tissue cleared with the LifeCanvas SHIELD...

11 March 2020 9,893 1 View

Is it possible to enhance DAB staining with heavy metals after mounting?

I have been working to optimize an HRP/DAB staining protocol for neuronal reconstructions in my lab for some time, now, and have consistently had issues with staining of distal dendrites. Our...

30 May 2016 5,355 5 View

Is the Surveyor assay able to detect large deletions?

I was hoping to use the Surveyor assay from IDT to detect large (~600bp) deletions in a gene of interest. The wt amplicon should be ~1.2kb. My main questions are: 1) Is it even likely that the wt...

30 May 2016 5,268 4 View