I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?

To analyze the growth inhibition of Candida albicans using OD600 measurements, a robust statistical model is crucial. While Logistic and Gompertz curves are common for growth data, their inability to yield satisfactory results indicates the need for alternative approaches. The nonlinear regression analysis method in GraphPad Prism is highly recommended [2]. This approach allows you to fit complex biological data using models such as the Richards growth model, which can accommodate different lag phases and growth rates.

Additionally, considering the Area Under the Curve (AUC) is useful for summarizing overall growth, but may not capture specific effects on lag phase or growth rate [3]. Therefore, combining nonlinear regression with AUC analysis can provide a comprehensive evaluation.

For precise fragmentation of gDNA, enzymes like HpaII and MspI can provide predictable cuts [1], facilitating more accurate and reproducible quantification in downstream applications.

In summary, use nonlinear regression models like the Richards model in GraphPad Prism for detailed growth curve analysis and consider AUC for overall growth assessment. Testing restriction enzymes such as HpaII and MspI will improve detection and quantification accuracy.

References:

[1] Dorazio, R., & Hunter, M. E. (2015). Statistical Models for the Analysis and Design of Digital Polymerase Chain Reaction (dPCR) Experiments.. Analytical chemistry, 87 21, 10886-93 .

[2] Mavrevski, R., Traykov, M., Trenchev, I., & Trencheva, M. (2018). Approaches to Modeling of Biological Experimental Data with GraphPad Prism Software. WSEAS Transactions on Systems and Control archive, 13.

[3] Chen, H., Zhou, X., Ren, B., & Cheng, L. (2020). The regulation of hyphae growth in Candida albicans. Virulence, 11, 337 - 348.

Hello Park Sowon,

Thank you for your quick response. The information you provided seems very useful. I will need to look at it in more detail and try it out in Prism. I will let you know if it worked!