Spatial thinking and geographical reasoning: what are their conceptual differences and interrelations in teaching geography?

I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.

17 January 2021 1,123 3 View

How do i interpret this inconsistent mediation in my results ?

Dear all and any, I have run a mediation analysis on SPSS using the PROCESS extension (model 4). My results don't really make any intuitive sense, where my ID (availability of healthy fast foods,...

09 January 2021 7,812 2 View

Does anyone know methodological references with techniques for random allocation (randomization) in Qualitative Research?

If that makes any sense....

03 October 2020 3,845 9 View

In indirect ELISA, how much recombinant protein should be add per well in 96 well?

19 September 2020 449 3 View

Tukey or Multiple t-tests ?

Hello everyone. I am conducting an experiment to evaluate if a compound inhibits a parasite growth quickly or slowly. To do this, I measure the IC50 of this compound when it acts for 24h, 48h or...

07 September 2020 5,361 5 View

Why do I have multiple peaks in my melt curve?

In the primer validation, with the serial dilution of the cDNA, I have a melt curve with a single peak. However, when I do the gene expression using control and treatment samples (I am evaluating...

17 August 2020 2,793 5 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

How to quantify percentage of DAB positive cells in IHC? Is there any automated tool?

Hi! I'm analyzing some TMA IHC cores using an scoring method that combines intensity and proportion of stained cells. I would like to know if is there any protocol to quantify percentage of DAB...

25 April 2020 479 2 View

Deep Euthetic Solvents, how to prepare?

Hi, everyone. I am starting to work with fractionation of Biomassa by Deep Euthetic Solvents . I came across a DES that is comprised of Lactic acid: Glucose:water (molar ratio 5:1:3), as in ref....

12 March 2020 3,474 3 View

What is the header of the Gaussian input file for calculating two distinct molecules?

I'm doing simulations with two different molecules, but it always ends up falling into the same problem, it seems that the Gaussian doesn't balance the molecules when I calculate the cation and...

03 March 2020 7,750 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View