Depends on which cells you want to grow. I've grown lymph node B cells before in the AIM-V media (Thermo fisher). If you wish to grow endothelial cells, blood or lymphatic, you can use the endothelial cell growth median MV2 (Promocell). If you wish to grow fibroblasts you can use fibroblast medium (Sciencell). All growth supplements are optimised in these media ready to go right off the bat. But I don't know a medium that was suitable for all lymph node cells
Tommy Lu thank you , and there is a growth factor or induction in case the cancer is low grade ? and your method is suitable to produce stable cell line ?