How do you find out the most cited paper from Web of Science?

Finding most citated papers in a given topics by using Web of Science tool

05 June 2019 2,765 4 View

No title

08 September 2018 3,685 4 View

How much medium is required to deactivate 1 mL trypsin?

As I know from researchgate question & answer session; "Split ratio depends on the volume of trypsin + medium to deactivate trypsin + cell" In this regards, my question is this statement correct?...

06 July 2018 1,666 0 View

Either anionic or non-ionic detergent is suitable for preparing lysis buffer?

I am presently planning to do western blot. In addition, I am working H9C2 cell lines. So, I need to prepare sample from cell line. Before to start, I have to prepare lysis buffer. In this...

05 June 2018 5,779 5 View

What are the shelf-life and optimal storage conditions for cell culture media?

I am currently working with H9C2 cell lines. I prepared the complete growth media by using following: 1. DMEM 2. 10% FBS and 3. 1% PS Finally, I know media (DMEM) by itself is generally stable for...

05 June 2018 9,877 4 View

What is the optimal storage condition of Sodium fluoride (NaF)?

In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...

05 June 2018 2,763 4 View

How to prepare the Fixer solution?

I need a complete protocol to prepare fixer solution for X-ray film development. Note: For western blot Enhanced chemiluminescence (ECL) detection method.

04 May 2018 9,812 0 View

How to know the cell H9C2 is fully differentiated?

31 December 2017 5,637 5 View

Best method for superoxide dismutase measurement ?

I am presently working with H9C2 cell. Now, I want to quantify superoxide dismutase enzyme after exposure of hypoxic condition to H9C2 cells. So, which methods should I have to use?

11 December 2017 8,789 1 View

How can I choose best methodology to determine apoptosis?

In particularly, I want to know what criteria should I have to consider before selection of a apoptosis methodology? In addition, I am presently working on Ischemic reperfusion induced apoptosis...

11 December 2017 6,257 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View