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Questions related from Dewan Sumsuzzman
Finding most citated papers in a given topics by using Web of Science tool
06 June 2019 2,881 4 View
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent. During subculturing, to detach the cell from the flask/dish and to avoid cell to cell adhesion...
09 September 2018 3,806 4 View
As I know from researchgate question & answer session; "Split ratio depends on the volume of trypsin + medium to deactivate trypsin + cell" In this regards, my question is this statement correct?...
07 July 2018 1,794 0 View
In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...
06 June 2018 2,865 4 View
I am presently planning to do western blot. In addition, I am working H9C2 cell lines. So, I need to prepare sample from cell line. Before to start, I have to prepare lysis buffer. In this...
06 June 2018 5,897 5 View
I am currently working with H9C2 cell lines. I prepared the complete growth media by using following: 1. DMEM 2. 10% FBS and 3. 1% PS Finally, I know media (DMEM) by itself is generally stable for...
06 June 2018 10,003 4 View
I need a complete protocol to prepare fixer solution for X-ray film development. Note: For western blot Enhanced chemiluminescence (ECL) detection method.
05 May 2018 9,917 0 View
I don't know whether this cell line H9C2 is suitable to do the next experient, because i don't know whether H9C2 cell is fully differentiated, could any expertise help me? any test methods?
01 January 2018 5,738 5 View
I am presently working with H9C2 cell. Now, I want to quantify superoxide dismutase enzyme after exposure of hypoxic condition to H9C2 cells. So, which methods should I have to use?
12 December 2017 8,860 1 View
I am presently working with H9C2 cell. Now, I want to quantify glutathione peroxidase enzyme after exposure of hypoxic condition to H9C2 cells. So, which methods should I have to use?
12 December 2017 6,112 2 View
Let me know more details about its composition. How it works? And its application?
12 December 2017 9,834 1 View
According to the abcam protocol, during, measurement of caspase-3 activity we need reaction buffer. So, I want to know what it is composition and basic mechanism of Caspase 3 Assay?
12 December 2017 492 1 View
At first, I need a complete protocol on it. Secondly, I want to know that, how much this assay effective in case of when I want to determine the antioxidant capability of irisin and melatonin...
12 December 2017 9,526 2 View
12 December 2017 2,112 1 View
In particularly, I want to know what criteria should I have to consider before selection of a apoptosis methodology? In addition, I am presently working on Ischemic reperfusion induced apoptosis...
12 December 2017 6,322 2 View
What is the composition of Wipe buffer?
11 November 2017 1,531 0 View
What is conference paper? What are the differences between a conference paper and journal paper?
11 November 2017 2,940 1 View
Uncoupling proteins (UCPs) belong to the superfamily of mitochondrial-inner-membrane anion-carrier proteins. Uncoupling proteins play a role in normal physiology such as it generate heat.
11 November 2017 9,679 3 View
Why DMSO(Dimethyl sulfoxide) is used in (PCR) polymerase chain reaction?
10 October 2017 5,287 4 View
What is Cryoprotectant? Please provide me research specific answer specially its applicability to cell culture.
10 October 2017 8,140 4 View
I need a specific comparison Between them.
10 October 2017 4,090 3 View
How can I differentiate these two buffer system?
09 September 2017 8,961 3 View
For western blotting, the primary antibody incubation time is very crucial. And, the incubation time depends on the binding affinity of the antibody and abundance of protein. I would like to know...
09 September 2017 4,028 2 View
The datasheet gives us valuable instruction which is very important to complete our experiment successfully. But, sometimes datasheet does not provide everything, for example, primary antibody...
09 September 2017 8,872 3 View
I want to know, 1. The standard formulation of blocking buffer. 2. Optimize storage condition. 3. Once prepare it, how long I can use it? 4. How blocking buffer reflect the final result?
09 September 2017 9,156 0 View
According to their application, how can I differentiate between TBS & PBS? As a blocking buffer which one is the best? When? Why?
09 September 2017 6,833 5 View
If gel percentage 15, 12, 10, 7.5, 5 (%) respectively, so what will these gel percentages optimum voltage and time?
08 August 2017 2,947 5 View
A number of methods had been developed for estimating protein concentration. Which method is most suitable in this case, and why?
08 August 2017 1,092 6 View
I know the particular application of BSA & Milk. But, my query, why many laboratories follow the combination of BSA+Milk as a blocking reagent? And, how they get advantages from this combination?
08 August 2017 1,927 3 View
Formulation ingredients as given below: 250 mM Tris base 1.92 M glycine 1 SDS D.W Many researchers said that room temperature is best for storage this SDS running buffer, but others argue. So, I...
08 August 2017 8,380 1 View
Generally, 4°C overnight will give more efficient and specific staining. However, many antibodies will also work very well with one or two hours incubation at room temperature. Actually, I need a...
08 August 2017 2,736 7 View
Definition? How to characterize them? Which software (free) is the best to analyze these outcome measures? How to combine Dichotomous outcome Continuous outcome (If data for the same outcome are...
01 January 1970 4,458 3 View
Focused on your formative years, How to describe your life philosophy?
01 January 1970 681 0 View
Which country most often recruit Systematic Review Data Analyst/Scientist? Thanks in advance, those who discuss future career perspective of this issue Experienced scholars are highly appreciated...
01 January 1970 937 1 View
