Hi there,

I'm wanting to lyse e.coli cells that I've previously treated with [1mM] IPTG and froze at -80 degrees yesterday. I was thinking to resuspend the pellet in lysis buffer to obtain the RNA I need. Does anyone have a recommendation on lysis buffer I should use? I plan on additionally treating with DNase and an RNase inhibitor to protect the RNA.

Thanks for your help!