Charlotte Campbell

4 Questions 3 Answers 0 Followers

Questions related from Charlotte Campbell

How to quantify how much of a fluorescent dye is entering a cell?

I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...

22 January 2021 6,270 3 View

Why does my plasmid grow when transformed into DH5a competent cells but not BL21?

I have a custom plasmid which expresses mCherry and will act as a reporter. I can transform this plasmid into DH5a and BL21. With DH5a I get a good number of singular colonies, but with BL21 I get...

03 September 2020 9,285 3 View

Best lysis buffer for IPTG treated bacterial cell pellet?

Hi there, I'm wanting to lyse e.coli cells that I've previously treated with [1mM] IPTG and froze at -80 degrees yesterday. I was thinking to resuspend the pellet in lysis buffer to obtain the...

27 February 2019 8,120 0 View

What is the best way of isolating and purifying RNA from DNA plasmid?

I have an aptamer within a DNA plasmid that I use for fluorescence experiments. When transcribed into RNA, the aptamer has a specific fluorophore it interacts with. I'm wanting to get Kd data of...

18 December 2018 9,275 6 View