Hi All,

I have B16F10 cell lines that I kept them in (-80) for about 2 weeks before I transferd them to liquid nitrogen , is it ok to still use these cell lines ? because i have read that ususally people keep them (24-48) hrs at -80 then from there they transfer them to liquid nitrogen, so I am not sure that two weeks at (-80) how much would affect on these cell lines and their behaviour? let me know if anyone have an idea about that

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