What's the best fluorophore to use for KI67 analysis?

I would like to check proliferation of human CD8 cells (isolated from blood ) after few days of culture. I have a panel of antibodies and I want to add KI67 to analyse proliferation. Does anyone...

08 October 2020 4,763 3 View

How can we test size and shape of nanoparticle?

There are many ways of measuring directly or indirectly the particle size at the nanometer scale, how can we test the nanoparticle size with good precision?

23 June 2020 139 28 View

Why nanotechnology is unique?

Nano is unique: properties, design and synthesis, applications, technology Nanotechnology is a common frame that integrates all disciplines: almost all fields have interest in nano (physic,...

24 May 2020 368 17 View

How we can create pores on metal oxide nano particles surface to enhance light capturing inside metal oxide NPs?

In order to achieve maximum hot electrons or lattice vibrations, is there any possibility to create pores on the surface of metal oxides. can we assume low acoustic impedance of materials under...

13 May 2020 7,235 4 View

Which is the concise introduction textbook for Nanotechnology course?

I'm looking for a very good textbook on Nanotechnology that can be given for the final year BSc students in physics and materials science could anyone suggest a book?I

12 May 2020 9,265 12 View

Which length do we take as the characteristic length of an aircraft to calculate Reynolds number and y+ value ?

An aircraft is consist of many sub-parts , while estimating the Reynolds number and y+ value first layer height which length do we consider as the characteristic length to be used in the...

15 March 2020 7,998 4 View

Is it possible to do an advanced shape optimization using adjoint solver without the add on of MRF ?

If one has do to some really complex shape optimization for the external aerodynamic problems . Is the adjoint solver has the enough capabilities to do the job without having command on MRF add on...

05 March 2020 6,825 1 View

Suggest me databases where i can find maximum studies regarding knee osteoarthritis??

I have to write a systematic review and I need maximum studies regarding knee osteoarthritis patients please suggest me all relevant databases.

22 February 2020 4,836 2 View

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I have added my article successfully but filed to be found using google search using any phrase combination.

25 January 2020 857 2 View

Why pressure far-field require a domain as far as 20 times of the characteristic length ?

Hello Everyone , Pressure far-field boundary condition is often recommended for flows with varying density , but why does it require so larger outer domains. Regards Aamir Sultan

10 January 2020 8,845 3 View

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02 March 2021 3,060 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View