I would like to use a "natural" axon based co culture model to study remyelination and lysolecithin induced demyelination. However, I do not have access to some popular cells lines that are used for this purpose: dorsal root ganglion explants, spinal cord explants, and motoneurons.
Would SH-SY5Y neuroblastoma cells from human bone marrow (https://www.atcc.org/products/all/CRL-2266.aspx ) be able to play the role of the axon when co-cultured with s42 (https://www.atcc.org/products/all/CRL-2942.aspx )schwann cells from the sciatic nerve of rats?
I plan to differentiate the SH-SY5Y cells with retinoic acid to make them more neuronal like.
I would appreciate any suggestions as to common cell lines that I could use for this remyelination and lysolecithin induced demyelination model and option for differentiating cell lines to be more neuronal like.
Thanks in advance!
Do you think it would be possible to study myelination and demyelination without a neuronal coculture (therefore, axons)? I am using s42 Schwann Cells which myelinate without needing to be provoked.
In the past, I treated s42 Schwann cells with ascorbic acid (which is meant to increase myelination according to several papers) and tested for Myelin Associated Glycoprotein (MAG) through and ELISA. It was expected that as the concentration of ascorbic acid increased, MAG would increase. However, the exact opposite occured. As the concentration of ascorbic acid increased, the amount of MAG decreased. (The concentrations of ascorbic acid I used are 0 M, 0.005 M, 0.01 M, 0.02 M, 0.04 M). I am not sure if this is because I did not coculture with neurons, which therefore does not give the schwann cells an axon to attach onto...
Thanks in advance!
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