Will that be stable enough ? how can we check for its stability and efficacy?
Hello Satish Kanna
It should be stable enough as the period of 16-18 hours at 24-26 degree C is not too long a period to affect the stability of FBS. You would want to refer to the article attached below for more information on the stability of serum at various temperatures and time points.
Article Amino Acids and Ammonia of Fetal Calf Serum During Storage.
Some of the methods that you could use to check the efficacy of FBS would be as follows.
The Plating efficiency in which you could assess the ability of serum to facilitate cell adherence to solid surfaces for growth. You could plate cells at low density in media supplemented with 5-10% FBS and monitor for attachment and clonal growth after 10-14 days.
Another testing method would be the cell expansion and viability method in which you could evaluate the nutritional support of FBS which could be typically evaluated on both adherent cells as well as cells grown in suspension.
If not much difference is observed in the expansion and viability of cells using both the FBS (FBS stored at the normal storage temperature and FBS stored at 24-26 degree C for 16-18 hours), then you could conclude that there has not been much change in the efficacy of FBS being kept at 24-26 degree C for 16-18 hours.
Best.