We are looking for a device that can purify bacterial genomic DNA for downstream PCR applications. Should process one or more (96 max) samples at a time. Good cost/efficiency ratio a plus.
you can try Mag-Bind® Bacterial DNA 96 Kit .The Handbook as below attachment
Hi Francois, check the application note attached. You may find it attractive. Cost is most efficient whether you work 96 samples in a (50 min) run. You may allso purify 1 sample, but this is not convenient. High throughput instruments a meant for big number of samples, up to 24 you better use manual methods. If you have medium throughput you want a smaller instrument, also presented in the note. This one is special by its post-elution capabilities. If you run >50,000 samples per year, the cost per isolation could be as low as 2$. Walk away.
Great, thanks. Is there a scientific paper that compares several apparatus from different companies?
No, it compares 2 devices from the same company for the universal pathogen isolation protocol on various sample types, such as sputum, CSF, urine, swabs, feces, whole blood, serum, difficult samples. If you need a more comprehensive comparison please let me know. The data are provided by a German lab.
What kind of samples? Do you just need to break open bacterial cells and get rid of the debris or do you need to purify the DNA from clinical/environmental samples?
Samples are overnight cultures in 1 mL of Letheen broth. A variety of microorganisms may be present and do not represent pure cultures. Thanks Hannah.
I am also looking for an answer to this. Same boat, looking for fully automated where the entire process is handled by the machine. Everything I have found includes a prepwork to lysis the cells which can take up a decent amount of time. Qiacube, FisherKing Flex, EpMotion . . . all dissapointing that this has not been made fully automatic for bacterial cells from pure culture.
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