Hannah Tanner

4 Questions 19 Answers 0 Followers

Questions related from Hannah Tanner

How can I get the ribosomes in a total nucleic acid extraction?

I need to do total nucleic extraction from fungi out of clinical samples. At the moment we are using bead beating followed by a total nucleic extraction protocol on the Roche MagNA pure compact....

24 April 2015 6,660 3 View

What are the top tips for optimising AFLP like fingerprint PCRs?

I have a PCR which generates multiple PCR products of different sizes from bacteria with one primer pair in one reaction (PCR-ribotyping). The PCR products are between 150 and 600bp but I'm...

04 February 2014 239 6 View

Has anyone got any experience with the Prove-it system from Mobidiag?

I have just read the Laakso and Maki paper in MicrobiologyOpen 2013; 2(2):284-292 and wondered if anyone has any experience with this system that they could share with me. I'm particularly...

15 July 2013 8,801 0 View

RNAse and Proteinase together?

I'm extracting genomic bacterial DNA in reasonably large quantities and I need it to be high quality too. The final step in the protocol I have been given incubates overnight in TE with 0.5% SDS,...

19 February 2013 7,527 9 View