Guanfan Mao

3 Questions 9 Answers 0 Followers

Questions related from Guanfan Mao

How do you create a codon preference file in the bio software DNAMAN?

I need to do Codon-optimized in the reverse translation part.

19 November 2014 3,561 0 View

Optimization Real Time PCR analyze for GC% Rich gene

Sometimes the whole gene region contains high GC content[~80%],it is difficult for Real Time PCR to analyze the simples;I have tried to design several primers to optimizate the results,but they...

24 December 2012 4,495 5 View

Can Real Time PCR analyze high homologous genes?

Sometimes two genes contain high Homology[95~98%],it is difficult for Real Time PCR (SYBR Green method)to design primers,and it seems not possibile to distinguish the Major gene .Can somebody...

24 December 2012 294 3 View