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Sometimes the whole gene region contains high GC content[~80%],it is difficult for Real Time PCR to analyze the simples;I have tried to design several primers to optimizate the results,but they...
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Sometimes two genes contain high Homology[95~98%],it is difficult for Real Time PCR (SYBR Green method)to design primers,and it seems not possibile to distinguish the Major gene .Can somebody...
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