If your microplate reader has a luminescence capability, then you can use it. Most multimodal plate readers can be used for luminescence, but absorbance-only plate readers can't. Your samples should be in white plates for maximal sensitivity, although black plates can also be used. Clear plates may not be usable because of well-to-well crosstalk.

"I am using the same kit for ATP determination in cell lines. Can any one explain how to do lysis these cells after treament and estimation of ATP from these cell lines"

Some agent that immediately inactivates all ATPases:

https://www.researchgate.net/post/Which_is_a_good_Tissue_Cell_Lysis_Buffer_for_Luminescent_ATP_assay

ATP can degrade in a matter of seconds during sample preparation. This is a challenging issue for animal tissues: with live animals the true [ATP] determination can get very tricky. For cell lines people often use trichloroacetic (TCA) or perchloric (PCA) acid solution which almost immediately inactivates everything when mixes with a culture medium. In some metabolomics papers 80% methanol and other solvents seem to work well: Article Metabolomic profiling of the heart during acute ischemic pre...

Hi all, i am using kit A22066 - molecular probe too to determin ATP in fungal spores. I am doing a standard curve but i need to know the time after adding the ATP standard solution to the reaction buffer before measuring om micro-plate. Thanks