Andrés Felipe Leal

5 Questions 3 Answers 0 Followers

Questions related from Andrés Felipe Leal

Mann-Whitney Test for MTT assays through Origin Pro 8 program?

Dear all!... I have done some assays of cell viability with MTT reactive and I would like to know if there are differences between un-treated and treated cells. Because the sample size is small,...

12 October 2019 1,779 0 View

ATP determination. Kit A22066- Molecular Probes?

Hi everyone!!... I´m doing an assay of cytotoxicity and I have a kit for ATP determination. However, I don't have a luminometer. It's possible, read in a conventional microplate reader?... Thanks. :)

26 October 2016 259 3 View

Griess Reaction for NO2 detection on J774A1 macrophages?

Hi everyone!... I'm trying detect NO2- on J774A.1 macrophages using Griess Reagent Kit for Nitrite Determination of Molecular Probes. However, I can't detect NO2-. I seeding 1e5 cells/well and 24...

19 April 2016 8,436 2 View

Which medium shall I use for LPS/INF-y preparation in activation assay of J774A.1?

Hi everyone! Is it necessary to use serum-free medium for prepare LPS/INF-y how positive control in activation assay with macrophages J774A.1 or can I use medium+FBS10%? Thanks.

17 January 2016 1,114 2 View

How can I harvest J774A.1 cells for flow cytometry?

Hi everyone!!... I need to use J774A.1 for flow cytometry but I don't know how can I harvest cells because I can't use trypsin. I seeding 1,5x105 cells/well in 24 well plate and incubate for...

16 December 2015 5,147 3 View