I need to know at which wavelength random coils , B-sheets , alpha- helix etc will appear in amide 1 of bone using raman . Lot of literature is there for FTIR but i couldn't find for RAMAN.
Maybe check these references
1. T. P. Pobezhimova and V. K. Voinikov, Membr Cell Biol, 2000, 13, 595–602.
2. I. Notingher, S. Verrier, S. Haque, J. M. Polak and L. L. Hench, Biopolymers, 2003, 72, 230–240.
3. I. Notingher and L. L. Hench, Expert Rev Med Devices, 2006, 3, 215–234.
4. A. Rygula, K. Majzner, K. M. Marzec, A. Kaczor, M. Pilarczyk and M. Baranska, Journal of Raman Spectroscopy, 2013, 44, 1061–1076.
Dear Sakshi,
in the past few years I've been working a lot on the determination of the secondary structure from IR and Raman spectra, reading a lot of literature. I prepared for a congress the following picture summarizing my literature survey: I hope this could help you!
Thank you sir. I found some good literature saying 1660-1672 there are turns but some says it is beta sheets in raman of amide I. can you please clarify?
It is true that there are mixed attribution; I usually consider that the range between 1667 ans 1675 is attributable to beta-sheet, while 1675-1695 is typical of turns.
Thank you sir. So, these are anti parallel Beta sheets. Can you please share with me any of the literature regarding this.
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Domenech J, Tinti A, Capdevila M, Atrian S, Torreggiani A (2007) FT-Raman and FTIR
approach to the structure of the Zn- and Cu-complexes of a type 2 plant (Quercus
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Lippert JL, Tyminski D, Desmeules PJ (1976) Determination of the secondary structure
of proteins by laser Raman spectroscopy. JACS 98:7075-7080.
Pelton JT, McLean LR (2000) Spectroscopic methods for analysis of protein secondary
structure. Anal Biochem 277:167-176.
Sane SU, Cramer SN, Przybycen TM (1999) A holistic approach to protein secondary
structure characterization using amide I Raman spectroscopy. Anal Biochem
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Schweitzer-Stenner R (2006) Advances in vibrational spectroscopy as a sensitive probe of peptide and protein structure. A critical review. Vibr Spectrosc 42:98-117.
Thomas GJ, Jr. (1999) Raman spectroscopy of protein and nucleic acid assemblies. Ann Rev Biophys Biomol Struct 28:1-27.
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