At what wavelength would you read a broth microdiultion plate at for bacterial growth (Salmonella)?

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How do I make up a 0.01M Na2HP04/NaH2P04 solution?

How do I make up a 0.01M Na2HP04/NaH2P04 buffer solution, pH adjusted to 7? Both chemicals that I have on hand are anhydrous. The molecular weight of Na2HP04 is 141.96 g/mol and the molecular...

08 September 2019 320 4 View

How do I keep Salmonella alive in a dry sample such as fish meal?

We often find Salmonella in fish meal (dry sample with around 6 % moisture and 9 % fat) samples but after a while (a week or two) the levels drop so low that we cannot detect it anymore. Is there...

05 June 2019 5,856 4 View

Mueller Hinton Broth: Cation-adjusted or Not?

I would like to use Mueller Hinton Broth to test for Salmonella growth and also separately for E.coli (Testing Minimum Inhibitory Concentration). Would I use a cation adjusted broth for these two...

04 May 2018 8,133 3 View

How long should you autoclave 5 liters of liquid?

How long should you autoclave 5 liters of liquid such as Buffered Peptone Water or Saline? We use room temperature distilled water and work on 15 minutes at 121 degrees Celsius for 1 Liter...

02 March 2018 5,539 0 View

Can someone recommend a bio-assay system?

Can someone recommend a bio-assay system? I am looking to test a variety of liquid antimicrobial agents against bacteria and moulds.

02 March 2018 4,567 9 View

How would you test the efficacy of a dry antimicrobial product?

How would you test the efficacy of a dry antimicrobial product (powder form)?. The dry product is applied to dry samples. I am trying to see which dosage works the best so I have to test different...

01 February 2018 897 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

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HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Masonry Interaction Module ABAQUS?

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How can we simulate(with FEM) the shape of Mobius strip using Abaqus Learning edition?

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15 July 2024 4,388 1 View

How to calculate CFU/m3 in settle plate?

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Badreddine Mechri

I hope that this article will be helpful. Best Luck.

Article Wavelength-normalized spectroscopic analysis of Staphylococc...

Aude Ferran

The most common is 600 nm.

Mert Sudagidan

Dear Nirvana, we prefer 600 nm to determine bacterial growth.

Sumon Dey

Dear, nirvana 600 nm is most preferable for bacterial growth because of maximum diffraction.