At what magnification we can see the cell wall of a bacteria and which microscope used the SEM or TEM ?

More Ikram Imene Bekkaye's questions See All

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Any suggestions to enhance the wettability and adhesion of fluorosilanes?

I am facing a difficulty to prepare the thin film which contains fluorine contained silica monomer, as we know F is highly reactive with low surface energy and it could be the reason of low...

09 July 2024 6,017 3 View

How to convert magnetic conductivity from SI to lattice units?

I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...

07 July 2024 5,243 0 View

How can I deal with the error message generated while dropping pdb files on Autodock tool ?

Dear Docker community, I'm a beginner at using molecular docking, and I'm facing a problem when I want to open or drop a pdb file on Autodock tools. The folder is saved with the extension.pdb;...

05 July 2024 8,348 4 View

Inquiry Regarding Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT)?

Dear All, I am writing to solicit your expertise regarding the application of Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT) for structural...

22 June 2024 7,751 5 View

Please tell me my research gate id?

please tell us my research gate id.

03 June 2024 3,623 0 View

Tumor xenografts in NOD/SCID vs NSG mice?

I am planning to create xenografts of solid tumor cells (sarcoma) in NOD/SCID mice. Our lab has previously established such tumors in these mice but with a certain cell type we have not been able...

17 March 2024 2,946 3 View

What could cause an decrease in the electrical resistance of a conductor when it is under cyclic sollicitations?

the metallic conductor is subjected to cyclic tensile-tensile loading, while the mechanical response clearly shows that the damage to the material increases as the number of cycles increases; it...

27 February 2024 8,079 0 View

What is the difference between active mode and inactive mode for RAMAN spectrophotometer analysis ?

Does active mode mean that there is a vibration and does the inverse meaning for inactive mode?

13 February 2024 8,150 0 View

Comment on peut suivre la décomposition de NaBH4 lors d'une réaction de réduction de 4-nitrophenol???

Comment on peut suivre la décomposition de NaBH4 en fonction de la masse du catalyseur

28 November 2023 654 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

David Eugene Booth

It probably depends on the size of the bacteria studied as well as the properties of your EM. Could you be a bit more specific. Thanks, David Booth

Aedrian Abrilla

If you are interested in the surface details of the cell wall, you'll likely need SEM. If you are interested in its cross-sectional details, you'll likely need TEM.

Ikram Imene Bekkaye

I am currently working with enterococci and lactobacilli, I am looking on the one hand to observe their cell form before and after stress and on the other hand to observe the cell wall before and after the same stress, what should I use? if I use the SEM can I use a liquid sample for the metallization or do I have to have a lyophilisate? At what magnification for each observation (shape / wall) Please!!