I'm looking to assess ATP concentrations in both red blood cells and blood plasma. I understand that the luciferase assay is relatively more straightforward than the HPLC assay and so am leaning toward this. Are there any particular considerations that need to be taken into account when using the luciferase assay (other than being careful to follow the assay protocol)?
Can anyone recommend any suitable assay kits? - there looks to be a number of 'brands' available!
Many thanks, Jack