Asking for a piggyBac cDNA cloning and expression vector?

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Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

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How can we now if the promotor in the vector is well?

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Transfection in HEK293T cells?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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Hanna Alalam

Maybe this with some modification will work for you?

https://www.addgene.org/92078/

Kaiming Li

Thank you, but the vector you recommend is not suit.

I've been searching for three days from addgene, but I haven't found the right one.

I see. have you considered ligating the promoter you want in one of the other available vectors?

Thank you for your advice