Hi all, i have some doubts about SH-SY5Y cell line. i don't know if you have ever met.
in the beginning, when i received the cells, i use the DMEM(ATCC) +10%FBS(gibco) to culture the cells, the cells grew well, and expanded a lot(look at the first picture).
but later, something happened to the cells, after 2 days when i subculture or thaw the cells, some cells detached and became big clumps(please look at the picture), i never met this situation, the sphere-shape cell still alive and metabolized, but it seems abnormal.
i tried to thaw two vials at the same time, use the same high glucose DMEM medium(gibco), use two brand FBS(gibco and BI), but the results are same, they began detach and became sphere after 2 days.
i hope someone can tell me the reason, thanks a lot.
https://www.researchgate.net/post/Why_do_my_SH-SY5Y_cells_clump_up_and_detach_from_the_plate
The RG discussion may be helpful. Good luck.
Actually, when i use 10% gibco FBS to culture the cells, it grows well in the flask with high seeding density.
thanks god! the initial problem solved, it is because of the BI FBS which is not suitable for the SH-SY5Y cell line.
but when i seeding the cells in the 96-well plate, which seeding density is 10000/well, 40000/well, or 100000/well, the cells grow in the beginning, but after 2 or 3 days, the cells congregated and become several big clumps, like the picture 2, a another problem appeared, did anyone meet this situation before?
i was trying to find the difference of flask and plate when cells grow......
Hi Ava Lan ,
I'm working with SH-SY5Y cell line for two months now and I'm trying to differentiate them with serum deprivation + ATRA treatment and BDNF.
I never managed to reproductibly differentiate this cell line while I tried many different conditions.
What happens is, within 24h to 48h of RA treatment almost all my cells dies and I have the same problem as you : cells are rounded, floating and sometimes with big floating clumps.
I'm using GIBCO FBS but I will definitely double check for that.
I hope you found the problem, if you have a solution please come back to us and tell us what did you change in order to work.
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