I haven't personally used percoll but it is meant to have the same function as histopaque or ficoll. They are all used for density gradient centrifugation. I hope this helps.

Hi Amanda,

Both Percoll and Ficoll are same. And, we're using Lymphocyte Separation Medium (LSM) from brand MP biomedicals. So, almost all these has same method in principles.

I would suggest you go ahead with your technique.

Hi Amanda,

for PBMC isolation your  are different density gradients on the market. They use all high-mass, hydrophilic polysaccharide. Ficoll is a trademark of GE. There are other like Biocoll, Leucoprep or Lympho-Spin. They all work simelar but differe in price and yield :-). Best Michael