Are total ERK levels stable in HEK293 cells?

20 April 2020 0 7K Report

I'm reading this Article Reduction of protein phosphatase 2A (PP2A) complexity reveal...

article and it is not clear to me why they normalize to total ERK.

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No correlation between the independent variables and the dependent variable?

I want to conduct a multivariable linear regression analysis with 15 independent variables, however when I was checking the assumptions not a single IV correlates with the dependent variable. Can...

14 May 2024 5,151 7 View

How much PCR product do I have to use for a Cas9 digestion?

I needed to do a Cas9 digestion of the amplicon, but I did not achieve it. Some protocols refer to the use of 1-2 uL of PCR product directly with optional purification, but I do not understand why...

16 September 2023 3,526 0 View

How to interpret the strength of association of the biserial correlation (rb)?

Hello, I estimated the biserial correlation between an artificial dichotomous variable and a continuous variable. But I want to know how to interpret the result in terms of strength of...

13 September 2023 2,528 0 View

A bug in SPSS and jamovi?

I was preparing a seminar and accidentally stumbled upon an odd situation. When there is a negative relationship between the predictor (Extraversion) and the dependent variable (Depression...

12 June 2023 7,476 13 View

How is the slurry applied on the mesh current collector ?

Electrode preparation / mesh current collector / battery / Active material

18 April 2023 2,679 2 View

Is it possible to work with a liquid cell in SEM, if yes how? Is there a special liquid-cell holder for it ?

SEM/ Liquid-cell

18 April 2023 6,611 3 View

What is the difference between a liquid cell and a battery ?

Liquid cell Vs Battery

13 April 2023 3,555 3 View

Can I reinstated zoological nomen nudum (invalid name) to be valid?

Recently, I published two new spider species in Forests and registered two new species in Zoobank after the paper was published. However, the World Spider Catalog currently contains the...

22 March 2023 3,201 2 View

How does one determine the sex of soft ticks (Argasidae)?

Sex in hard ticks (Ixodidae) is very easy to determine, but does anyone have a reliable method to determine the sex in soft ticks (Argasidae)? Thanks in advance. Manuel

16 February 2023 5,866 2 View

Is the in-situ raman pouch cell used only once ? and cannot be disassembled and assembled again for a second use ?

Unlike the ampix cell used for in-situ operando, can we disassemble the pouch cell and the coin cell and assemble them for a second use ?

20 January 2023 5,048 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View