Luna Moon

4 Questions 1 Answers 0 Followers

Questions related from Luna Moon

Is it better to compare AUC or IC50 values against Protein Expression Data?

I'm using the proteomics data set from DepMap and I have the AUC and IC50 values for compounds from GDSC...what is the best method to compare? I used AUC and relative protein expression to...

20 May 2020 8,805 0 View

How can I find the correlation coefficient between 1 categorical variable compared to several other variables?

Let's say that I have 8 proteins: A-H and I have protein expression data for each gene. For each gene, the expression number is based on the average of 20 cell lines expressing that gene. I also...

11 May 2020 3,479 1 View

Are total ERK levels stable in HEK293 cells?

I'm reading this Reduction of protein phosphatase 2A (PP2A) complexity reveal... article and it is not clear to me why they normalize to total ERK.

20 April 2020 6,885 0 View

Can anyone clarify the difference between IC50 and dose to me?

I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I...

25 February 2020 7,293 2 View