Why do we use Agarose gel for DNA and SDS PAGE gel for protein?

I am completely new in the field of molecular biology or gene cloning. so i am learning lot of new laboratory techniques recently. Can someone tell me why we can not use Agarose gel for proteins?

02 March 2019 9,666 17 View

Which sections of liver and brain are most suitable to study autophagy process?

I learned recently, that autophagy may have a protective role against the development of a number of neurodegenerative diseases. As I am working on liver diseases, with mice deficient for some...

02 March 2019 4,025 3 View

Does anyone know what exactly determines the choice for a buffer in organs homogenates?

Because it seems like it depends on the laboratory, but i really like to know if buffers (Tris, Phosphate, Krebs or McEwen) are not specific to a type of organ If yes, what is this specificity...

09 October 2015 8,191 2 View

Is anyone working on Nymphaea lotus or other Water Lily species?

I am working on the androgenic and aphrodisiac properties of the aqueous extract of the flowers of N. lotus, but I know that this plant is a widely spread plant, so I don't understand why no one...

03 April 2014 7,953 9 View

How long do the effects of a chronic administration of L-Name last in male rats?

I conducted an experiment to see the effects of high blood pressure on erectile function of albino male rats. I based my protocole on the fact that effects of L-name are reversible after...

03 April 2014 989 2 View

Does someone have the procedure to perform a toluidine blue coloration after the strips have been fixed?

I have used Formaldehyde 10% to fixed rats organ after an experiment and I will make a post-fixation in bouin liquid before performing trimming and staining, but I don't know if it is compatible...

03 April 2014 7,680 4 View

What tests can I use to study the aphrodisiac properties of a product in vivo, on a Wistar male rat without using oestrous female rats?

I am working on different models of erectile dysfunction and I used to induce oestrus chemically in Wistar female rats, but they are less responsive to the induction and takes too much time (eye...

02 March 2014 312 10 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View