I have used Formaldehyde 10% to fixed rats organ after an experiment and I will make a post-fixation in bouin liquid before performing trimming and staining, but I don't know if it is compatible with toluidine blue stain. And I really don't know how to proceed, because I don't have any protocols to perform this stain.
Hello, What cells are you looking for? The toluidine blue is for mast cells, correct? We have a lot of histology stains and their solutions posted on our website at http://histologistics.com/histology/special-stains/
I have also attached the protocol, make sure you run a positive control (skin works well). I hope this helps!
Thank you so much, i want to identify myelinised axons on cavernous nerve fixed strips.
Is this on brain tissue? If so then have you though about doing luxol fast blue staining?
I normally use the Toluidine blue stain in plant tissues thus: we fix samples in FAA (50% ethanol+5% formaldehyde+10% glacial acetic acid, in water), then embed samples in paraffin to obtain think sections (using a microtome). After that, we apply 0.05% Toluidine blue (TBO) in PO4 buffer during 5–10 min. Finally, we remove paraffin to observe samples in the microscope.
This process is very effective.
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