Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

How to analyze data from an augmented design for locations x genotype interaction?

I have data of multiple environment trials (MET) , the design used was an augmented design in each of the locations. And I would like to analyze the data with Rstudio or a free software. Does...

06 November 2020 6,084 3 View

Does anyone know of an open access journal indexed in JCR with no publication fee?

I wonder if anyone knows a dream journal that meets all the following 3 criteria: indexed in the reputable Journal Citation Reports open access publication free of charge Our research falls...

23 January 2019 2,084 10 View

Do I need to purify labeled primers to sequence microsatellite with a fragment analyzer?

To detect microsatellites using primers labeled in applied biosystems platform. I will use multiplexing (3 dyes) same specie. I need to make it as cheap as possible, in sequencing equipment (serie)

01 September 2018 4,539 3 View

Why would you use a non-parametric test such as Kruskal-Wallis test instead of a parametric test such as ANOVA?

We are conducting a research in the field of Behavioral Finance where some of the papers that we consulted have used the Kruskal-Wallis test for testing mean differences among groups. However we...

15 August 2018 1,385 4 View

Aside from M9 Minimal Media, what are other types of minimal media?

My partner and I are trying to culture bacteria. We are attempting to culture bacteria in minimal media using plastic as its carbon source. We would like to know an alternative in case we do not...

07 May 2018 2,413 3 View

Does anybody knows if HEK293T cells express either Mannose Receptor or Mannose 6 fosfate receptor?

Does anybody have experience in cell protein internalization assays using this cell line?

18 August 2017 2,510 1 View

Are there any other tracking methods aside from satellite tagging that are just as efficient in tracking the long distance movements of whale sharks?

The migratory patterns of Rhincodon typus is very interesting to me and I have read that satellite tagging is the method typically used when tracking their movements. However, I would like to know...

07 July 2016 3,382 2 View

Can someone answer my questions on Fluent dynamic mesh?

I am currently doing a 2D simulation of a rotary positive displacement pump which uses the wankel mechanism (used by the wankel engine). Some questions I have are 1. for dynamic mesh, why does it...

03 February 2016 1,947 2 View

What is the best agar to use to isolate enterococcus?

I am trying to build a library of known fecal isolates using antibiotic resistance assays, but I am having trouble isolating enterococcus using enterococcal agar. Any suggestions?

21 January 2016 5,177 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View