I want to indentify fungus isolated from beans.
@ Edgar, my answer is NO. Although the amplication size may vary strain to strain but with ITS1 and ITS 4 primers, you should get an amplicon with size ranges between 500-700 bp.
Thank Dr. Tarafdar. I used a microwave protocol for DNA extraction, and DNA for amplification of ITS r, but my amplicon reached 1000pb
We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments
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Attached is the structure in question
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How can I save the list of publications to an external location? Best regards Edgar Selzer
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How to design VN primer to attach with universal reverse primer
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Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
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Please discuss someone told about fungus and allied others.
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