Is posible that primers its 1 and its 4 amplify a fungus fragment > 950 pb?

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J. C. Tarafdar

@ Edgar, my answer is NO. Although the amplication size may vary strain to strain but with ITS1 and ITS 4 primers, you should get an amplicon with size ranges between 500-700 bp.

Edgar Guevara Avendaño

Thank Dr. Tarafdar. I used a microwave protocol for DNA extraction, and DNA for amplification of ITS r, but my amplicon reached 1000pb